Accelerated Oxidation Test of Freeze-Dried Pseudomonas Fluorescens BTP1, BB2 and PI9 Strains

Authors

  • Mputu Kanyinda Jean-Noel Walloon Center for Industrial Biology (CWBI), Bio-Industry Unit, Gembloux Agro-Bio Tech, University of Liege, Passage des Déportés, Gembloux Belgium; Department of Chemistry, Faculty of Sciences, University of Kinshasa, Kin XI, DR Congo
  • Karl Tshimenga Department of Chemistry, Faculty of Sciences, University of Kinshasa, Kin XI, DR Congo
  • Noki Philippe Department of Chemistry, Faculty of Sciences, University of Kinshasa, Kin XI, DR Congo

DOI:

https://doi.org/10.18488/journal.33/2015.2.2/33.2.18.26

Abstract

Pseudomonas fluorescens strains are very susceptible to damage during freeze-drying and storage. It would be useful to have longer lasting viable powders. Membrane oxidation is one of the factors responsible for damages suffered by P. fluorescens strains during storage. Singlet oxygen (1O2) is a highly reactive form of molecular oxygen that may harm living systems by oxidizing critical cellular macromolecules. We exposed freeze-dried powders of three strains P. fluorescens (BB2, BTP1 and PI9) to singlet oxygen produced by photosensitisation of Methylene Blue/ light during 2 hours. We observe a significant loss in survival rate, a pronounced oxidation of polyunsaturated fatty acids, protein oxidation (carbonyl groups) and protein insolubilisation. This study enables better predictions of types of packaging suitable for freeze-dried powders of P. fluorescens.

Keywords:

Accelerated oxidation test (AOT), Carbonyl group, Freeze-drying, Oxygen singlet, Pseudomonas fluorescens, Reactive oxygen species (ROS)

Abstract Video

Published

2015-06-15

How to Cite

Jean-Noel, M. K. ., Tshimenga, K. ., & Philippe, N. . (2015). Accelerated Oxidation Test of Freeze-Dried Pseudomonas Fluorescens BTP1, BB2 and PI9 Strains. The Asia Journal of Applied Microbiology, 2(2), 18–26. https://doi.org/10.18488/journal.33/2015.2.2/33.2.18.26

Issue

Vol. 2 No. 2 (2015)

Section

Articles