Effects of Degasified Extender on Quality Parameters of Cryopreserved Bull Spermatozoa

Authors

  • Megha Pande ICAR-Central Institute for Research on Cattle, Meerut Cantt, Uttar Pradesh, India
  • N Srivastava ICAR-Central Institute for Research on Cattle, Meerut Cantt, Uttar Pradesh, India
  • J.S Rajoriya Division of Animal Reproduction, ICAR-Indian Veterinary Research Institute, Izatnagar, Bareilly, Uttar Pradesh, India
  • S.K Ghosh Division of Animal Reproduction, ICAR-Indian Veterinary Research Institute, Izatnagar, Bareilly, Uttar Pradesh, India
  • J.K Prasad Division of Animal Reproduction, ICAR-Indian Veterinary Research Institute, Izatnagar, Bareilly, Uttar Pradesh, India
  • S.S Ramteke Division of Animal Reproduction, ICAR-Indian Veterinary Research Institute, Izatnagar, Bareilly, Uttar Pradesh, India

DOI:

https://doi.org/10.18488/journal.110/2015.1.3/110.3.70.78

Abstract

One of the most important factors contributing to poor quality semen post thaw has been reported to be oxidative stress. Continued interaction of spermatozoa with dissolved oxygen in extender renders detrimental effect by generating reactive oxygen species during processing. This formed the basis to investigate the effects of removal of oxygen from the Tris-Glycerol-Egg Yolk (TG-EY) extender on the post-thaw semen quality. Semen ejaculates (n=26) collected from three cross bred bulls with mass activity of ≥3 and progressive motility of ≥70% were split in two fractions (control and treatment) to obtain 80 x 106 progressively motile sperm/mL of extended semen. The TG-EY extender composed of 3.028 g of Tris, 1.675 g of citric acid, 1.25 g of fructose, 7.0 mL of glycerol, 10 mL of egg yolk, 105 IU Penicillin G Sodium and 105 µg Streptomycin for 100 mL of deionized water for control and degasified (freeze-pump-thaw cycling method using liquid nitrogen) for treatment. Semen was processed and filled in 0.25 mL polyvinyl chloride straws, sealed and cryopreserved for at least 2 week before evaluation. Assessment of post-thaw motility, viability, acrosome and plasma membrane integrity of spermatozoa were evaluated after thawing at 37°C for 30s. Study revealed a significant (viability and acrosome integrity, p<0.05) and highly significant (post-thaw motility and plasma membrane integrity, p<0.01) improvement in all the semen quality parameters in degasified as compared to control group. This study showed that degasification of extender prior to their use can serve to improve semen quality to highly acceptable levels.

Keywords:

Oxidative stress, Acrosome integrity, Cryopreservation, Plasma membrane integrity, ROS, Motility

Abstract Video

Published

2015-09-30

How to Cite

Pande, M. ., Srivastava, N., Rajoriya, J., Ghosh, S., Prasad, J., & Ramteke, S. (2015). Effects of Degasified Extender on Quality Parameters of Cryopreserved Bull Spermatozoa. International Journal of Veterinary Sciences Research, 1(3), 70–78. https://doi.org/10.18488/journal.110/2015.1.3/110.3.70.78

Issue

Section

Articles

Most read articles by the same author(s)