https://archive.conscientiabeam.com/index.php/57 Conscientia Beam en-US The International Journal of Biotechnology 2306-9864 https://archive.conscientiabeam.com/index.php/57/article/view/4224 <p>Bacterial leaf blight is a major threat to rice production worldwide, especially in Bangladesh. The best approach for plant breeders to address this issue is to develop resistant varieties using the available resistant gene pool. The current study used DNA marker technology to identify major BLB resistance genes in Bangladesh's landraces and local rice cultivars. We used reported molecular markers to assess the presence of BLB resistance genes (<em>Xa4</em>, <em>Xa5</em>, <em>Xa7</em>, <em>Xa13</em>, and <em>Xa21</em>) in genotypes. The results showed that the <em>Xa4</em> gene was present in 10 genotypes. Two of these genotypes also gave positive bands for the <em>Xa5</em> gene, while three showed positive results for the <em>Xa13</em> gene. Among these, one genotype contained <em>Xa13</em> and <em>Xa5</em> genes, and another had <em>Xa7</em> and <em>Xa21</em> genes. Additionally, two genotypes were found to carry both the <em>Xa7</em> and <em>Xa13</em> genes. The phylogenetic tree illustrates the genetic relationships among 41 rice genotypes, grouping them into four main clusters. Within these clusters, genetically similar genotypes tend to be grouped. This study offers valuable insights for identifying genotypes that carry multiple resistance genes, which could serve as a potential resource for breeding programs to develop rice genotypes resistant to bacterial leaf blight.</p> Kazi Meftahul Jannat Khadiza Khatun Md. Rafiqul Islam Mokaram Hanifa Koly Nazmun Naher Copyright (c) 2025 2025-05-23 2025-05-23 14 1 1 8 10.18488/57.v14i1.4224