Index

Abstract

A 2 by 4 experiment laid out in RCBD was conducted at Crop science and Horticulture agricultural farm, Nnamdi Azikiwe University, Awka, Nigeria. The experiments were conducted from June to September 2020 to study the effects of different rates (0.0, 0.1,0.2,0.3 t/ha) of wood ash on the growth and yields of carrot varieties in Awka South Eastern Nigeria, the experiment was carried out on pots and beds. It was observed that treatment 0.3 t/ha performed significantly better in most of the assessed parameters both on growth and yields such as: plant height, marketable yield, root diameter, whole plant biomass, root length, total yield and harvest index. While 0t/ha performed lowest in the growth and yield of carrot varieties for the bed experiment. On pot experiment, 0.2t/ha of wood ash gave highest result both on growth and yield parameters (harvest index, leaf fresh weight, marketable yield, root diameter, root fresh weight, root length, whole plant biomass, and total yield in the pot experiment). Then with 0.3t/ha of wood ash and carrotte touchon variety was the best combination for carrot yield on bed experiment. Generally, carrote touchon performed better than tonchon France carrot variety both in the pot and field. Field experiment was also the best experiment than pot experiment.

Keywords: Replication, Experiment, Field, Pot, Significant, Yield, Varieties.

Received: 6 December 2021 / Revised: 10 January 2022 / Accepted: 24 January 2022/ Published: 1 February 2022

Contribution/ Originality

This study is one of very few studies which have investigated how carrot responds to different rates of wood ash as growth promoter both in bed and pots.

1. INTRODUCTION

Carrot (Daucus carota) a biennal herbaceous specie is a member of the Apiacea family [1]. It belongs to the same family as parsnip, celery, parley, dill and fenel [2].The cultivated carrot are mainly classified into eastern carrots and western carrots based on pigmentation [3].The Eastern carrots are thought to originate from Afghanistan, while the origin of the Western carrot is still uncertain [1]. The roots of most western carrot are: orange, red or white. The leaves of western carrots are highly dissected and the roots are unbranched [3].The roots of eastern carrots are yellow and some are purple; They have slightly dissected leafs and branched roots; The orange carrots are popular and more widely cultivated in the world; Western carrots are always cylindrical or tapered cylindrical in shape and have less pubescent leafs [1]. Eastern carrots always have thicker, shorter, narrow, conical roots, have pubescent leaves and flower early [1]. Like many other coloured vegetables, carrot is a gold mine of antioxidants; they have been shown to have inhibition mutagenesis activity contributing to decrease risk of some cancers; Carrot also exerts anti carcinogenic activities, reduce inflammatory insult, and moderate immune responses [4]. Among 39 fruits and vegetables, carrots have been ranked 10th in nutritional value; Carrot is a good source of dietary fiber and of the trace mineral molybdenum, rarely found in many vegetables [3]. Comparing to other vegetables, carrots can provide in the human diet significant amount of vitamin A due to the high bioavailability of carrot carotenoids [3]. Also, carrot is particularly rich in carotene (pro- vitamin A) [5].

Wood ash is the residue powder left after the combustion of wood, such as burning wood in home fire place or an industrial power plant. It results from burning or gasifying wood and consists mainly of minerals that the trees have absorbed over their life time. Wood ash increases the pH, Ca, Mg, K and P of a brunisol and luvisol but has little effect on their microbial activity and biomass [6]. A study in Alberta has shown that lime and wood ash increased microbial biomass and C mineralization, and changed the functional structure of bacterial communities in an acid agricultural soil [7]. Also, Wood ash has been used extensively in northern Europe to improve forest productivity and to curtail the cost of landfills [7].

Persistent over use of fertilizers to increase the crop yield has led to serious consequences on environment and human health. Carrot yield and nutritional quality are affected by the fertilizers applied and the varieties Win [8]. Adekayode and Olojugba [9] suggested that the increasingly high cost of mineral fertilizers and preferred economic disposal of wood ash had necessitated research in the use of wood ash to reduce the rate of fertilizer application for maize production [10] Scientists are now on look for alternatives of fertilizers which can substitute nutrients in soil without damaging the environment; this led to the use of wood ash in the experiment instead of chemical fertilizer. Due to the chemical composition of wood ash, it has been shown that ash can be used to raise pH in soils. Its content of many of the  plant nutrients often limiting plant growth suggests that wood ash could also be useful as fertilizer; since  the content of nitrogen is low, fertilization with only wood ash would not be sufficient for most crops [11].Wood ash increased crop performance in production of crops such as Soybeans [12, 13] cassava [14], tomato and pepper [15], groundnut [16] and vegetables [17]. On other hand, the organic source of nutrients provide consistent and slow release of nutrients, maintain ideal C: N ratio, improve water holding capacity and microbial biomass of soil profile, without any adverse residual effects [18]. Although wood ash has been applied on crops at different rates, unfortunately, the Effect of Different Rates of Wood Ash on the Growth and Yield of carrot (Daucus carota) in Awka South - Eastern Nigeria has not been reported.
The objectives of the research were therefore to:

  1. Determine the effects of wood ash on the growth and yield of carrot.
  2. To identify the rate of wood ash that gives optimum yield on the production of carrot.
  3. To compare the performances on the field and pots.
  4. To compare the yields of the two cultivars.

2. MATERIALS AND METHODS

2.1. Experimental Site Description

This present experiment was carried out at the Department of Crop Science and Horticulture Research Farm, Nnamdi Azikiwe University, Awka, Anambra State Nigeria to study the Effect of Different Rates of Wood Ash on the Growth and Yield of carrot (Daucus carota) in Awka, South­Eastern Nigeria. Awka is characterized by tropical rainforest with temperature of 270C-300C.The area is located between latitude 06015lN and longitude 07008lE, with an average rainfall of 1810.3 mm per annum. The bed and pot experiments were carried out in the raining season (June – September, 2020).

2.2. Experimental Treatment and Experimental Design

This research was carried out on beds and pots. For the bed and pot experiment, 2 factors were used: Factor A=Wood ash rates (0, 0.1, 0.2 and 0.3 tonnes/ha); Factor B= 2Carrot cultivars. The experiments were a 2×4 factorial experiment laid out in Randomized Complete Block Design (RCBD) with 4 replications. These varieties were sourced from a reputable dealer at Jos. The wood ash rates 0, 0.1, 0.2 and 0.3 tonnes /ha and two carrot cultivars listed above were used for the two experiments. The treatment combination for the bed and pots includes: A0tonne/ha+V1, A0tonne/ha+V2; A0.1tonnes/ha+V1; A0.1tonnes/ha+V2, A0.2 tonnes/ha+V1, A 0.2tonnes/ha +V2, A0.3tonnes /ha+V1, A0.3tonnes/ha+V2.

2.3. Bed and Pot Experiment Description

The bed experiment was conducted to evaluate the effects of different rates of wood ash on the growth and yield of carrot in Awka, South- Eastern Nigeria. The field had a land area of 11.5m by 5.5 m and a plot size of 1m x 1m, furrow spacing of 0.5m along each plot and a 0.5m pathway across each plot. Plant spacing of 20cm by 30cm was used. A total of 16 seeds were sown per bed. On the whole 32 beds were used for the bed experiment. The pot experiment was conducted to evaluate the effects of different rates of wood ash on the growth and yield of carrot in Awka, South- Eastern Nigeria. A total of 32 pots were used as in the field for the experiment and were perforated at four spots at the base and four spots by the side to ensure effective drainage and aeration. Top soil was collected from the Department of Crop Science and Horticulture Research Farm, Nnamdi Azikiwe University, Awka, Anambra State, Nigeria. The soil was air dried for three days to expose pests and some disease-causing organisms.

2.4.Data Collection and Analysis

Soil samples from 0­15cm depth of the experimental site were taken by simple random sampling method in order to analyze the physicochemical properties of the soil at the start of the experiment. Ten sub-samples were randomly taken and mixed thoroughly to get a composite sample. The soil sample was then pretreated by air drying and passed through a 2mm sieve before analysis. Wood ash was also sieved to remove unwanted particles and taken to the laboratory for analysis. Plant height was measured in centimeters (cm) using a meter scale at 45,60 and 75 days after sowing (DAS) from the point of the attachment of the leaves to the root (ground level) up to the tip of the longest leaf. Number of leaves per sampled plant both for the bed and pot experiments was counted at 45, 60 and75 DAS. All the leaves of the plants were counted separately. Only the smallest young leaves at the growing point of the plant were excluded from the counting. The plant girths of sampled plants were recorded for the bed and pot experiments. A thread was placed at the base of each sample to measure its circumference and was placed on a meter rule. The plant girth was measured separately and recorded at 45, 60 and 75 DAS. The average diameter of the root was measured at the thickest portion of the root at harvest with the help of a slide caliper. The average length of the root was recorded in cm using a meter rule from the point of attachment of the leaves (proximal end) to the last point of the root (distal end) in each treatment combination. Total yield tonnes /ha was taken which consists of both good quality roots, branched, racked and rotten roots. The marketable yield of roots tonnes /ha which consisted of good quality roots other than branched, racked and rotten roots was measured. Thus: Marketable yield = Gross yield - Non marketable yield of cracked, branched and rotten root. Number of harvested plants per bed and pot was also measured where three plants were harvested from each bed and pot. Harvest Index on fresh weight basis (%) was collected by calculation using the formular below:

Table 1. Root uniformity indices and percentages.

S/No Index Description Percentage of each Index
1 Not Uniform <30% of the roots are similar in size and shape
2 Fairly Uniform >30%<50% of the roots are similar in size and shape 
3 Uniform >50%< 70% of the roots are similar in size and shape
4 Very Uniform >70%<90% of the roots are similar in size and shape
5 Highly Uniform >90% of the roots are similar in size and shape

Data were collected on root uniformity using visual examination of the roots following uniformity indices as shown Table 1.

For determining the significance between the treatment means and to draw valid conclusions, statistical analysis was made. The data from the various observations were subjected to statistical analysis by using Analysis of variance. The difference of the treatment means was tested using least significance difference at 5% level of significance and GENSAT version 12.1, year 2009 was used.

3. RESULTS AND DISCUSSION

3.1. Soil and Wood Ash Physico –Chemical Properties of the Experimental Sites

In Table 2, the available result indicated that potassium content in the wood ash was 4.99%, nitrogen 0.025%, organic carbon 1.26%, organic matter 2.17% while C:N was 45.00. Other parameters were also observed in the same table. The result of analysis carried out with soil samples collected from the experimental site as presented in Table 3, shows the physical and chemical soil properties. Based on the physical soil properties, there was high percentage of sand (71.2%), clay was the least (8.4 %) while the textural class of the soil is sandy-loam. The chemical soil properties showed the percentage base saturation to be 80.23 %, organic matter 1.98%, and Total N0.97%, other parameters were also determined.

Table 2. Wood ash chemical composition.

Properties
Percentage (%)
pH in water
10.69
Organic carbon (%) 
1.26
Organic matter (%)
2.17
Phosphorus 
0.317
Ca2+
19.58
Mg 
2.31
N (%)
0.025
K (%)
4.99
C: N ratio
45.00

Table 3. Physical and chemical properties of the soil samples taken at 0 – 15cm depth of the experimental site before planting.

Soil Properties
Percentage (%)
Physical
Sand (%)
69.6
Silt (%)
22.0
Clay (%)
8.4
Textural class
Sandy-loam (SL)
Chemical
pH (H2O)
5.14
Total N (%)
0.97
Organic carbon (%)
1.14
Organic matter (%)
1.98
Ca (Cmolkg-1)
2.27
Mg (Cmolkg-1)
1.33
K (Cmolkg-1)
0.25
Na (Cmolkg-1)
0.12
EA (Cmolkg-1)
1.63
ECEC (Cmolkg-1)
4.97
BS (%)
80.23
Available P (mgkg-1)
5.53

Abbreviations: P-Phosphorus, N-Nitrogen, Ca-Calcium, Mg-Magnesium, K-Potassium, Na-Sodium, EA-Exchangeable acidity, ECEC-Effective Cations Exchange Capacity, BS-Base saturation.

3.2. Effects of Wood Ash Rates on the Growth Parameters of Carrot Varieties (Daucus carota) For the Bed and Pot Experiments

In Table 4, for the bed experiment, the growth parameters of carrot such as plant girth, leaf number and plant height increased with an increase in the application of wood ash rates, but then no significant difference was recorded. This agrees with the work of Ajala, et al. [19] who reported that the growth parameters such as plant height, number of leaves, leaf area, leaf area index, leaf length, stem  diameter, number  of  flowers,  number  of  pods, weight  of  plant and  total biomass of amended maize-lima beans intercrop were significantly (P>0.05) increased by wood  ash  application, Lupwayi, et al. [7] reported that the tallest carrot plants were obtained with high levels of Shrawan, et al. [20] explained  that increased potassium increased the number of leaves per plant and  addition of ash to tilled and non- tilled soil increased cassava growth parameters (plant height, number of leafs and stem girth).

Table 4. Main effects and interactions of wood ash rates and variety on plant height and leaf number of carrot for bed experiment.

TRT
P H
LN
PG
 
W-Ash
45
DAS
60
DAS
75
DAS
45 DAS
60
DAS
75
DAS
45
DAS
60
DAS
75
DAS
 
T0
17.9
23.9
32.0
7.99
7.74
7.75
2.04
2.53
3.08
 
T1
14.8
22.2
28.5
5.12
6.36
7.87
2.18
2.68
3.12
 
T2
21.4
29.3
42.3
5.83
7.17
9.12
3.05
3.69
4.05
 
T3
19.8
31.2
42.4
4.83
7.42
9.80
3.07
3.57
4.04
 
LSD
7.64
8.98
8.36
3.956
1.761
2.329
1.386
1.555
1.415
 
Variety
V1
19.6
25.9
35.0
5.93
6.97
8.21
2.86
3.20
3.82
 
V2
17.3
27.4
37.6
5.96
7.37
9.07
2.31
3.04
3.32
 
LSD
5.40
6.35
5.91
2.797
1.245
1.647
0.980
1.100
1.001
 
INT                
 
T0×V1
18.9
24.1
30.1
8.31
7.06
7.25
2.43
2.91
3.41
 
T0×V2
16.8
23.7
33.8
7.67
8.42
8.25
1.64
2.15
2.75
 
T1×V1
12.9
18.1
21.9
3.98
5.42
6.75
1.77
2.27
2.65
 
T1×V2
16.6
26.3
35.1
6.25
7.29
9.00
2.59
3.09
3.59
 
T2×V1
24.7
28.2
44.0
6.42
7.25
9.08
3.86
3.72
4.86
 
T2×V2
18.0
30.4
40.6
5.25
7.08
9.17
2.23
3.66
3.24
 
T3×V1
21.8
33.2
44.1
5.00
8.17
9.75
3.38
3.87
4.37
 
T3×V2
17.8
29.3
40.7
4.66
6.67
9.85
2.76
3.27
3.72
 
LSD
10.81
12.71
11.82
5.594
2.490
3.294
1.960
2.200
2.001
 

Note: TRT=Treatment, PH= Plant height, LN= leaf number, PG= Plant girth, LSD=Least significant difference, INT=Interaction.

For the pot experiment Table 5, statistically, the main effects and interactions of wood ash rates and carrot varieties was not significantly different (P>0.05) as there was variations in growth parameters. This is not in line with the report of  Lupwayi, et al. [7] that the vegetative growth, including plant height, number of leafs, fresh and dry weight of leafs were enhanced by higher potassium levels while control plants produced the lowest values.

3.3. Effects and Interactions of Wood Ash Rates and Carrot Varieties on The Yield of Carrot (Daucus Carota) For the Bed and Pot Experiment

3.3.1. Bed Experiment

With the result of the bed experiment in Table 6, statistically, the main effects and interactions of wood ash rates and carrot varieties were significantly different (P>0.05) on the yield parameters. This is in consonant with the study of Mbah and Nkpaji [21] who stated that when synthetic fertilizers were not applied, the use of wood ash produced significant effect on the yield of many crops, particularly maize; [7] reported that wood ash application generally improved soil properties which in turn enhanced maize grain yield. Wood ash increased performance in production of crops such as Soybeans [12, 13] cassava [14, 22] tomato and pepper [15] groundnut [16, 23] and vegetables [17].

Table 5. Main effects and interactions of wood ash rates and variety on plant height and leaf number of carrot for pot experiment.

TRT
P H
LN
PG
W-Ash
45
DAS
60
DAS
75
DAS
45 DAS
60
DAS
75
DAS
45
DAS
60
DAS
75
DAS
T0
22.68
25.09
24.78
5.07
6.02
6.20
2.60
2.76
2.99
T1
25.88
27.55
27.02
5.40
5.35
6.00
2.39
3.01
3.22
T2
29.22
29.54
28.90
5.54
5.40
6.00
2.94
3.12
3.32
T3
25.96
27.36
25.59
5.52
5.27
7.50
2.79
2.99
3.19
LSD
5.608
5.090
5.343
0.628
0.809
1.033
0.883
0.749
0.723
Variety
V1
27.15
27.83
27.34
5.48
5.43
41.00
2.65
3.05
3.27
V2
24.72
26.94
25.80
5.28
5.59
5.00
2.71
2.89
3.09
LSD
3.966
3.599
3.778
0.444
0.572
73.0
0.624
0.530
0.511
INT                
T0×V1
23.46
24.53
24.52
5.33
5.54
6.00
2.59
2.78
2.97
T0×V2
21.90
25.65
25.05
4.80
6.50
6. 00
2.61
2.75
3.00
T1×V1
27.04
27.60
28.18
5.50
5.58
6. 00
1.99
3.02
3.28
T1×V2
24.72
27.51
25.85
5.29
5.12
5.00
2.80
3.00
3.17
T2×V1
30.34
30.41
30.17
5.50
5.71
6.00
3.00
3.20
3.40
T2×V2
28.10
28.67
27.63
5.58
5.08
5.00
2.88
3.05
3.25
T3×V1
27.75
28.79
26.49
5.58
4.88
146.
3.2
3.23
3.42
T3×V2
24.16
25.94
24.68
5.46
5.67
5.00
2.56
2.76
2.96
LSD
7.931
7.199
7.556
0.888
1.144
146.0
1.249
1.059
1.022

Note:  TRT=Treatment, PH= Plant height, LN= leaf number, PG= Plant girth, LSD=Least significant difference, INT=Interaction.

Table 6. Main effects and interactions of wood ash rates and variety on yield parameters of Carrot (Daucus carota) for bed experiment.

TRT
H I
LFW
MY
NOHR
RD
RFW
RL
RU
TMY
 
W-ASH
%
G
tones /ha
 
cm
G
Cm
%
%
 
T0
30.8
20.3
0.000024
3.00
1.24
8.5
15.5
54.2
3.57
 
T1
52.7
27.3
0.000059
2.38
1.60
28.2
19.0
68.8
2.83
 
T2
51.3
48.1
0.000121
2.75
1.84
51.5
22.4
70.8
3.27
 
T3
47.0
60.6
0.000136
2.71
2.41
53.5
23.2
54.0
3.22
 
LSD
20.02
20.47
0.0000722
0.681
0.716
22.02
6.44
22.87
0.810
 
Variety
 
V1
47.0
42.6
0.000113
2.75
2.06
38.9
22.0
58.3
3.27
 
V2
43.9
35.5
0.000057
2.67
1.49
31.9
18.1
65.6
3.17
 
LSD
14.16
14.47
0.0000510
0.481
0.506
15.57
4.55
16.17
0.573
 
INT
 
T0×V1
32.4
14.3
0.000021
3.00
1.25
8.1
15.9
50.0
3.57
 
T0×V2
29.1
26.3
0.000026
3.00
1.23
8.9
15.1
58.3
3.57
 
T1×V1
60.4
26.4
0.000088
2.00
1.71
28.0
18.6
75.0
2.38
 
T1×V2
45.0
28.1
0.000030
2.75
1.48
28.5
19.4
62.5
3.27
 
T2×V1
48.8
53.8
0.000150
3.00
2.26
51.2
26.6
58.3
3.57
 
T2×V2
53.8
42.3
0.000092
2.50
1.43
51.9
18.2
83.3
2.97
 
T3×V1
46.2
75.9
0.000191
3.00
3.01
68.4
26.9
50.0
3.57
 
T3×V2
47.7
45.4
0.000081
2.42
1.81
38.5
19.6
58.1
2.88
 
LSD
28.31
28.95
0.0001021
0.963
1.012
31.15
9.10
32.35
1.146
 

Note: H I= Harvest Index, LFW= Leaf fresh Weight, MY=Marketable Yield, NOHR=Number of Harvested roots, R D=Root diameter, RFW=Root Fresh Weight, RL=Root length, RU=Root Uniformity, TMY=Total Marketable Yield.

The result of the current study revealed that the application of 0.3t/ha of wood ash enhanced the following yield parameters: harvest index, marketable yield, root diameter, leaf fresh weight, root length, root fresh weight, total yield and whole plant biomass. This is in line with the work of Silva Dias [5] who stated that there is an increase in root yield, marketable yield in carrot root, juice concentration and total titratable acidity of root juice with increase in organic manure rates. The genotypes were significantly similar on the number of harvested roots for the pot and bed experiment. This was also stated by Aarssen [24] in his hypothesis that all variations of the competitive abilities among genotypes do not differ sufficiently among coexisting species to cause any competitive exclusion at the community level.

3.3.2. Pot Experiment

Table 7 showed the results of wood ash and variety effects on yield parameters of pot experiment. The parameters measured showed no significant in almost all the parameters. The parameters having no significant effect on the pot experiment could probably be as a result of environmental factors being almost homogeneous that is, same quantity of sand, water, same size of pot and only wood ash rates and carrot varieties were varied. This also means that the wood ash does not have any significance effect on the performance of carrot in the pot experiment. However, the combination of 0.2t/ha of wood ash and carotte touchon varieties enhanced plant height, harvest index, leaf fresh weight, marketable yield, root diameter, root fresh weight, root length and total yield in the pot experiment. Also the combination 0.3t/ha of wood ash and carotte touchon variety significantly enhanced the yield parameters of carrot in the bed experiment such as leaf fresh weight, marketable yield, root diameter, root fresh weight, marketable yield, root diameter, root length, total yield and whole plant biomass. This finding is in agreement with the report by Ojeniyi, et al. [25] which stated that addition of ash to tilled and non-tilled soil increased cassava growth.

Table 7. Main effects and interactions of wood ash rates and carrot varieties on yield parameters of carrot (Daucus carota) for the pot experiment.

TRT
HI
LFW
MY
NOHR
RD
RFW
RL
RU
 
W-Ash
%
g
tones /ha
cm
G
cm
%
 
T0
61.2
3.23
0.000028
5.875
1.513
6.5
10.75
29.2
 
T1
62.1
4.37
0.000033
6.000
1.723
7.4
10.61
33.3
 
T2
67.9
4.98
0.000086
6.000
1.871
12.6
11.00
25.0
 
T3
63.9
3.79
0.000030
6.000
1.646
8.5
9.13
33.3
 
LSD
8.56
2.635
0.0000617
0.1838
0.4868
6.33
3.748
13.28
 
Variety
V1
64.8
4.34
0.000059
6.000
1.738
9.6
10.45
32.3
 
V2
62.8
3.85
0.000030
5.938
1.639
7.9
10.29
28.1
 
LSD
6.05
1.863
0.0000436
0.1300
0.3442
4.47
2.650
9.39
 
INT
T0 ×V1
62.9
2.87
0.000030
6.000
1.625
7.5
10.87
25.0
 
T0×V2
59.5
3.59
0.000026
5.750
1.400
5.6
10.63
33.3
 
T1×V1
60.7
5.17
0.000034
6.000
1.665
6.9
10.76
37.5
 
T1×V2
63.5
3.58
0.000031
6.000
1.780
7.9
10.46
29.2
 
T2×V1
71.7
6.09
0.000150
6.000
2.063
17.1
12.23
25.0
 
T2×V2
64.2
3.88
0.000023
6.000
1.680
8.1
9.77
25.0
 
T3×V1
63.9
3.25
0.000021
6.000
1.598
7.0
7.95
41.6
 
T3×V2
63.9
4.33
0.000039
6.000
1.695
10.0
10.31
25.0
 
LSD
12.10
3.726
0.0000872
0.2600
0.6884
8.95
5.301
18.79
 

Note: TRT= Treatment, LFW=Leaf fresh weight, MY= Marketable yield, NOHR= Number of harvested roots per bed, RD= Root diameter, HI= Harvest Index, RFW= Root fresh weight, RL=Root length, RU=Root uniformity, LSD= Least significance difference.

4. CONCLUSION

Considering the overall result, the combination 0.3t/ha of wood ash and carotte touchon variety was the best option for carrot yield of bed experiment while the combination 0.2t/ha of wood ash and carotte touchon variety to an extent was the best option for carrot yield of pot experiment.

To obtain maximum yield for carrot production, carrot seeds should be grown on beds and not on pots. Carotte touchon variety should be used and 0.3t/ha of wood ash should be applied. If pots are to be used, 0.2 t/ha of wood ash should be applied.

Funding: This study received no specific financial support.  

Competing Interests: The authors declare that they have no competing interests.

Authors’ Contributions: All authors contributed equally to the conception and design of the study.

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