Phylogeny of Trichoplusia ni (Lepidoptera: Noctuidae) 18S and 5S ribosomal RNA gene sequence

Abstract

Trichoplusia ni Hübner, a cabbage looper belonging to the Noctuidae family of Lepidoptera, is a highly varied agricultural pest. It is essential to use partial sequences of nuclear DNA, 18S, and 5S ribosomal genes to isolate and analyze the phylogenetic relationships within T. ni and other insect species families and orders using distinct gene markers. T. DNA. After being extracted and sequenced using primers, Clustal W alignment and NCBI BLAST were completed. Members of the 18S BLAST lineage sequences are classified according to the order Coleoptera and the family Coccinellidae. A total of 2461 positions are occupied by the 33 sequences that were chosen for the final dataset. In the final dataset, 19 nucleotide sequences totaling 1935 positions were chosen from the taxonomy asset of 5S BLASTed lineages, which included members of the order Hymenoptera and the family Chalcidoidea. For both genes, phylogenetic analyses were performed using the Maximum Composite Likelihood model (ML), which aids in the construction of phylogenetic relationships at various taxonomic levels of trees, provides phylogenetic insights for understanding relationships between closer clades, and explains some significant regions of substitution rates appropriate for changing evolutionarily distant taxa.